GOF Recipe cookbook:
• Human specificity
• Cell Entry
• Animal to Human transmission
• SARs 1.0 backbone
• MERs mechanism
• H5N1 : HA glycoprotein via Furin
• SCH004 Bat to Human entry
• RS Baric
• Fort Derrick, UNC Chapel Hill, July 2019
• 7th CISM Military World Games, Wuhan 2019, October 18–27, 2019
“Acquisition of the furin cleavage site might be viewed as a ‘gain of function’ that enabled a bat CoV to jump into humans and begin its current epidemic spread.”
“Strikingly, the 2019-nCoV S-protein sequence contains 12 additional nucleotides”
These are the “insertions” of genetic engineering.
Prof. Francis Boyle of the University of Indiana who drafted the 1989 Biological Weapons Act considered Menachery etal.’s SHC014CoV or the 2019-nCoV as “an offensive biological warfare agent, and it has no legitimate scientific, or medical use of the Gain-of-Function (GOF) technology”
According to Boyle, GOF was a DNA genetic engineering technology of “turbo-charging” dangerous biological warfare substances or pathogens.
Furins are known to control infection by avian influenza A viruses, in which cleavage of the HA glycoprotein is needed for entry into the cell
Insertion of a furin cleavage site in the HA of highly pathogenic avian H5N1 influenza viruses leads to replication in multiple tissues and higher pathogenicity, due to the distribution of furins in multiple tissues.
Proteolytic cleavage of the S glycoprotein can determine whether the virus can cross species, e.g. from bats to humans. For example, the S glycoprotein from a MERS-like CoV from Ugandan bats can bind to human cells but cannot mediate virus entry.
However, if the protease trypsin is included during infection, the S glycoprotein is cleaved and virus entry takes place.
This observation demonstrates that cleavage of the S glycoprotein is a barrier to zoonotic coronavirus transmission.
Examination of the protein sequence of the S glycoprotein of SARS-CoV-2 reveals the presence of a furin cleavage sequence (PRRARS|V).
The CoV with the highest nucleotide sequence homology, isolated from a bat in Yunnan in 2013 (RaTG-13), does not have the furin cleavage sequence. Because furin proteases are abundant in the respiratory tract, it is possible that SARS-CoV-2 S glycoprotein is cleaved upon exit from epithelial cells and consequently can efficiently infect other cells.
In contrast, the highly related bat CoV RaTG-13 does not have the furin cleavage site.
Meanwhile, it is possible that the insertion of a furin cleavage site allowed a bat CoV to gain the ability to infect humans. The furin cleavage site might have been acquired by recombination with another virus possessing that site.
There are 12 warfare bases housing many BSL3/BSL4 laboratories specialized in system engineering of biological warfare weapons in the USA, and the BSL3/BSL4 warfare laboratories of Fort Detrick in Chapel Hill, North Carolina was one of them.
According to the news report, Fort Detrick had its license revoked last July 19 after an outbreak similar to COVID-19, killing about 20,000 people with 75% of nearby citizens infected.
And then we had the World Military Games in Wuhan, known as the 7th CISM Military World Games and commonly known as Wuhan 2019, was held from October 18–27, 2019
And that is when all the fun began
Dr. Won CM
Bill Gates Sponsored Event 201 simulated pandemic in October 2019
The Washington Times Anthony Fauci should explain '$3.7 million to the Wuhan laboratory' Virology Journal Furin cleavage site in the SARS-CoV-2 coronavirus glycoprotein